This is a list of characters in Alex Basics in Biology and Zoology.
|Olivia||Olivia is the main protagonist. She is trapped inside Alex's school and is forced to collect notebooks in order to escape.||Human|
|Alex||Alex is the main antagonist. He will begin to chase you if you get an answer wrong, and if he catches you, he will show you his true form.||Human/Demon|
|Dolly||Dolly is the school's principal. She will lock you in the detention room if she spots you breaking any of the "RULEZ".||Living Ragdoll|
|Ms. Immortal||A strange shadow being who holds a blue broom. Roams the school and makes sure everything is clean.||Ghost|
|Scrina||Scrina is a flying robot with a monitor for a head. She is extremely insecure about people calling her a "thing", and she takes her anger out on others.||Robot|
|Asmodena||Asmodena is a small girl who wears a Pusheen-cat shirt. She loves to play games and will force anybody she sees to play them with her.||Human|
|Candice||Candice, also known as Candy princess, is a fairy creature who flies around Alex's school. She loves candy, or anything else sweet, and will try to consume any she sees.||Human/Fairy|